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Description
Human CACNA2D1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against the calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of calcium voltage-gated channel auxiliary subunit alpha2delta 1 (CACNA2D1) in the sample. The absorbance (OD value) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Calcium voltage-gated channel auxiliary subunit alpha2delta 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Voltage-gated calcium channel α2δ1 subunit (CACNA2D1) is a protein encoded by the CACNA2D1 gene. This gene encodes a member of the α-2/delta subunit family and is a protein in the voltage-dependent calcium channel complex. Calcium channels mediate calcium influx into cells upon membrane polarization and are composed of α-1, α-2/delta, β, and γ subunits in a 1:1:1:1 ratio. This protein is considered the molecular target of gabapentin and pregabalin, which are used to treat epilepsy and neuropathic pain. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.3 ★★★★★
Based on 1917 reviews
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Product Reviews
★★★★★ 5
EXACTLY as advertised and MORE! HIGHLY recommended! 😊
Scent: Unscented
I've been using this product for 2 years and it works as stated. I have sensitive skin and this doesn't irritate my skin at all.
Every so often, I've asked those close to me if they've noticed any odors over the past two years and they've always replied no. I've used other brands previously that were specifically labeled as "gentle" or for "sensitive skin", which were anything but. Those were also HIGHLY scented and irritated my skin, unlike this product which is very effective without all the unnecessary chemical additives. I feel the coconut oil has soothing properties and it actually helps keep my underarm skin healthy instead of making it parched, like other brands do.
This product doesn't state that it's for sensitive skin but for me it's far more gentle than any other brand I've used. I also like that it doesn't contain any harsh anti-perspirants so my body can breathe and cool itself off properly through the natural act of sweating. The act of sweating releases toxins through your skin pores so it's not healthy to impede that process. This product supports that natural process by not containing any anti-perspirant chemicals.
Although it's labeled as unscented, science will tell you that everything in the world has a scent (even water) and this product has a very minimal wisp of a scent that's nearly imperceptible. Manufacturer's usually refer to this as a 'masking scent' so you don't notice the smell of chemicals in a product. However, this product has minimal chemicals (that's why I chose it) so there's not much to cover up and that's a good thing!
Neither I, nor anyone else that I've asked, can detect a fragrance. The ONLY time I can smell anything is when I apply it to my underarms, so the product is obviously in closer proximity to my nose. As I glide it across my skin a faint scent of a Lifesaver's pineapple flavor candy takes me immediately back to my happy childhood memories. It lasts for a few seconds and then it's gone. Could just be me but every time I use this I end up with a smile on my face. 😊
I think this happens because your underarm skin is warm so the deodorant also warms and the solidified coconut oil melts a bit as you apply it, which releases the barely-there scent. I don't smell coconut, I smell glorious pineapple! Whoever thought to make it like this is genius! For those of you who don't smell anything for those few seconds, you're missing out.
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Reviewed in the United States on January 16, 2026
★★★★★ 5
Best natural deodorant
Scent: Coconut & Vanilla
I discovered Native Deodorant through an excellent rating on the Yuka app and decided to give it a try. I’m glad I did. The formula contains naturally derived ingredients, which was an important factor in my decision.
What impressed me most was how effective it is. It provides reliable odor protection throughout the day without irritation, and the application is so smooth and lightweight that it’s practically unnoticeable once applied. There’s no sticky or greasy feeling, and it feels comfortable from the moment it goes on.
Overall, this has been one of the best natural deodorants I’ve used. It combines clean ingredients with excellent performance, making it an easy product to recommend.
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Reviewed in the United States on June 5, 2026
★★★★★ 5
Good Natural Deodorant smelling fresh
Scent: Eucalyptus & Mint
I switched to Native after wanting to move away from aluminum-based deodorants and I've been really happy with it. The eucalyptus and mint scent is clean and crisp without being overpowering — it smells fresh in the morning and you can still catch a hint of it later in the day.
The stick goes on smoothly without that waxy drag some natural deodorants have, and I haven't had any white marks on dark shirts. Odor control has held up well through a normal workday and light activity. It's not an antiperspirant so you'll still sweat, but the scent stays pleasant.
No irritation for me even after the first few days, which is usually when baking soda formulas would get my skin angry. Overall a solid everyday deodorant — would buy again.
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Reviewed in the United States on May 26, 2026
★★★★★ 4
Great Performance, Just Not My Favorite Scent
Scent: Citrus & Herbal Musk
I’ve been using this Native deodorant for a while now, and overall it’s been a pretty solid option, especially if you’re trying to stick with something more natural. It goes on smooth, doesn’t feel greasy, and I like that it doesn’t leave behind a bunch of residue on clothes. It also holds up well throughout the day—definitely reliable for everyday use.
One of the big positives for me is the ingredient list. It’s aluminum-free and made with simpler, naturally derived ingredients, which is something I’ve been trying to prioritize more. It still manages to control odor effectively without feeling harsh on the skin, which isn’t always the case with natural deodorants.
As for the scent, it’s… fine. The citrus and herbal musk combo is clean and not overpowering, but it’s not my favorite either. I don’t dislike it, but I also wouldn’t go out of my way to pick this particular fragrance again—just comes down to personal preference.
Overall, it’s a dependable deodorant that works well and feels good to use. If the scent hits for you, it could easily be a go-to.
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Reviewed in the United States on April 24, 2026
★★★★★ 5
Love Native
Scent: Buttercream & French Vanilla
Great deodorant that’s aluminum free. Love all the Native deodorants. Helps keep body odor at bay. Goes on smooth and doesn’t tug on skin. If switching from an aluminum deodorant to this, give your body some time to adjust. Purchase all kinds of different scent and love them all. May leave some white marks on shirts if shirt rubs against your underarms.
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Reviewed in the United States on May 29, 2026
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