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Description
Human C5aR ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. 3. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Complement component 5a receptor (C5aR). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Complement component 5a receptor (C5aR) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Complement component 5a receptor ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Complement component 5a receptor 1 (C5aR1), also known as CD88 (cluster of differentiation 88), is a G protein-coupled C5a receptor. It functions as a complement receptor. The C5a receptor regulates inflammatory responses, obesity, development, and cancer. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.7 ★★★★★
Based on 921 reviews
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Product Reviews
★★★★★ 5
Finally found my endgame mouse
Color: Classic Black, Color: Classic Black
Finally found my endgame mouse — perfect for lightweight lovers and RTS gamers.
I've gone through a lot of mice over the years — office mice, productivity mice, various gaming mice — and I kept running into the same problem: they were either too heavy or too bulky for my taste. The Orochi V2 is the first mouse where I thought, "this is it."
The weight and size are just right if you prefer a light, nimble feel in your hand. It doesn't fatigue your wrist over long sessions, and the compact form factor gives you great control without feeling like you're pushing a brick around your desk.
If you play StarCraft 1 or other real-time strategy games that demand fast, precise movements, I genuinely think this is one of the best options you can find on Amazon. The responsiveness and accuracy make a noticeable difference when every click counts.
Battery life is also excellent for a wireless mouse, and switching between Bluetooth and the 2.4GHz dongle is seamless. Overall, this is the mouse I keep coming back to — and I don't see myself switching anytime soon.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 25, 2026
★★★★★ 1
Very glitchy mouse won't accept clicks, made me reinstall windows
Color: White
TLDR: Ask ChatGPT about this mouse and it'll tell you that other users have reported issues with this mouse not clicking properly. It will tell you that this is a known issue with this mouse.
So I'm an IT expert (programmer, been using PC's since Windows 3.1). And I could not get this mouse to work right. The mouse doesn't work right when the computer goes to sleep, and then wakes from sleep. This problem occurs on Windows 11 pro.
When the mouse is connected in Bluetooth mode: First problem is, the mouse will NOT reconnect to the PC when the PC wakes from sleep, unless you have "Show notifications to connect using Swift Pair" disabled in windows Bluetooth settings. But setting is on by default. Second problem is, even with this setting disabled, and even if the mouse successfully reconnects when the computer wakes up, the computer will NOT accept clicks when it wakes up. This mouse apparently drops a phantom click that it never releases. The only way I could find to get rid of that phantom click is by pressing CTRL+ALT+DEL every time the computer wakes. This resets the phantom click and the mouse would function normally. But it's super annoying to have to press CTRL+ALT+DEL every time the PC wakes, especially since this mouse is supposed to be a gift to my little girl, I don't want her to have to deal with it not working perfectly. Also the mouse would sometimes stop accepting clicks even in the middle of playing games, but this only happened rarely in Bluetooth mode.
On the wireless USB mode: The mouse cursor is NOT visible when windows first boots. You can't use the mouse to get to the box to type your PIN number to unlock the computer. You must use the keyword to get into windows, and then the cursor appears. This problem doesn't happen when using the mouse in Bluetooth mode, only in the wireless USB mode. And then the phantom click issue is MUCH more frequent in the wireless USB mode, and doesn't require the computer to wake from sleep, but happens randomly even in the middle of playing games... suddenly you can't click anything. And when waking from sleep, or when the mouse "goes to sleep", when it wakes up there's issues with clicks... and sometimes the cursor isn't even visible.
Connecting a second mouse: When the computer stops accepting clicks, then even the second mouse would not be able to click. All connected mouses would stop being able to click. But this problem of not being able to click, would ONLY occur if the Razor Orochi V2 mouse was connected to the computer either via Bluetooth or wireless USB. If I disconnected the Orochi V2 mouse, then the problem never happens. So it's definitely this mouses's fault.
But initially I didn't realize this mouse was the culprit. Because it affected all connected mice. So I thought, at first, that it was a windows issue... maybe a bad driver I had installed in the past. So I ended up reinstalling windows, trying to fix any potential driver issues. It wasn't until after that, when I discovered that the problem was this mouse all along. I was super upset to have reinstalled windows, and lost all my software and settings, only to then discover that the problem was still there and it was this mouse all along.
As for getting my money back, amazon did not offer me an option to return the mouse and get a replacement. It only lets me send it back for a full refund. No option for replacement. So I think I bought this mouse when it was on sale. So it seems like they are expecting me to send it back, and then repurchase it at the new higher price, if I want to try it again. It's possible mine was simply defective.
I did every imaginable thing to try to get this mouse to work. The mouse I had was brand new from Razor, but it was running an old firmware. I did a firmware update to version 1.03.00 (it was running version 1.02.00 when it arrived). I can confirm that firmware update did NOT fix the problem with the phantom clicks.
I also set all the related devices in my device manager to not go to sleep, including the USB slots. That did NOT fix the problem with the phantom clicks.
I also, at the advice of chatGPT, did a bunch of registry edits, none of which fixed the problem.
I also, at the advice of chatGPT, wrote my own c# application, which attempted in various ways to force the mouse to release the phantom click, at least when the computer would wake up. I was trying to mimic the behavior of pressing CTRL+ALT+DEL. After many hours of coding, I was unable to programmatically correct the problems with this mouse.
I really wanted this mouse to work because it's wireless, and its small, so it fits my daughter's hand.
I'm now having to return this mouse at my time and expense, no option for replacement, lost whatever discount I got from the previous sale, and I lost days of my life trying to fix this mouse, and I lost some of my software and settings from the reinstallation of windows, so I have work to do just to get my daughter's computer back to normal again. Terrible experience.
Conclusion: Ask ChatGPT about this mouse and it'll tell you that other users have reported issues with this mouse not clicking properly. It will tell you that this is a known issue with this mouse.
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Reviewed in the United States on November 7, 2025
★★★★★ 5
Works flawlessly for drafting use...
Color: Black, Style: Wireless, Pattern Name: Mouse
This mouse has been a great upgrade for my setup. The wireless connection is fast and reliable, and I honestly can’t tell the difference from a wired mouse. The sensor is very responsive, and everything feels smooth and precise, especially combined with the Logitech G mouse pad.
I actually use this all day at work for drafting, so precision and comfort really matter, and this mouse delivers on both. I’ve even purchased several more for my drafting team, and everyone has been really happy with it.
I also like the customizable weight system—you can adjust it to feel just right in your hand. Overall it feels solid, well made, and comfortable for long hours of use.
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Reviewed in the United States on March 17, 2026
★★★★★ 5
This is the last mouse you will ever buy
Color: Black, Style: Wireless, Pattern Name: Mouse
Just buy it.
What can be said about this mouse that hasn't been said before? This is the perfect mouse, there is no contest. I know it is a bit on the pricier side, but if you need a mouse, just get the cheapest one you can get and start saving to get this one, IT'S THAT GOOD.
The mouse glides over any decent mouse pad, you don't need to get a Logitech one. The shape is perfect for all kinds of grips and the weight system is great for customization.
The led has different brightness levels, including turning it off completely to save more battery, and the keys are just silent and satisfying to press.
Again, this is the best mouse ever, no other mouse will offer a better value for your money than this one. A bit steep, yes, but worth it.
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Reviewed in the United States on March 20, 2026
★★★★★ 5
One of the best mice I've ever owned
Color: Black, Style: Wireless, Pattern Name: Mouse
I was a little skeptical about this mouse but after 3 weeks of use it's continued to be my favorite mouse. Not only does it. Have a good feel. It is very durable and customizing. The weight is fantastic. If you get the charging pad it just glides. But is definitely worth its money
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 19, 2026
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